By Madison Vain
Updated October 16, 2015 at 12:53 PM EDT
Credit: Douglas Gorenstein/NBC

Jimmy Fallon’s entrance to the stage on Thursday night got an a cappella makeover by Pentatonix. The Grammy-winning vocal group performed the show’s theme song, originally by the Roots, to welcome Fallon to the stage. And in the show’s pre-roll, you can barely tell the music is the work off all human harmonies, which is sort of the selling point of the Los Angeles group.

Pentatonix was also last night’s musical guest. They performed their current single, “Can’t Sleep Love” off their self-titled debut full-length. Check the performance out below.