By Mandi Bierly
Updated September 01, 2010 at 07:57 PM EDT

Actually, it’s Zach’s twin brother, Seth Galifianakis, who has the honor of interviewing the Oscar winner in the latest installment of Between Two Ferns. Though Penn shoots glorious daggers at Galifianakis throughout the sitdown, only once does he verbally threaten him: “I’ll knock you the f— out right in your chair.” And surprisingly, this isn’t when “Seth” nominates Ryan Reynolds as the true movie star of our time, or when he asks Penn about his work in Haiti and interrupts him to ask if there’s a Six Flags down there (then moves on when Penn answers no). It comes after Seth takes offense to Penn’s lack of enthusiasm at the suggestion that he, Penn, and “Jack Nicklaus” go to Long John Silver’s and talk about putting Seth’s pug, Funyuns, in a movie. Watch below.